www.fluorescencemicroscopy.it

Fluorescence Microscopy...
and other Techniques

by Augusto Sciarra

This site aims to describe the world of Fluorescence Microscopy, a technique widely used, both in medicine and biology, to observe the characteristics of specific biomolecules such as cellular proteins labeled, antigen-antibody complexes, chromosomes, individual genes, cellular components, etc.
This technique, however, is less known and used by the amateur microscopist due to its complexity, the difficulty in obtaining materials needed for sample preparation and the particular type of microscope to use. The aim of the site is to disseminate the knowledge base to allow the use of Fluorescence, even by "non experts", to this end will, therefore, will be highlighted the ways, the substances and the type of subjects which best provide observation, will be considered, therefore, bacteria, tissue histology, algae, microorganisms, cells, etc.. more easily available from the amateur microscopist.
The site, in addition to fluorescence, will also examine the main techniques in contrast microscopy, will be discussed, therefore, of Darkfield, Polarization, DIC, Bright field, Rheinberg, Phase contrast, COL.
For each of these techniques will describe the basic concepts, the characteristics, the type of optical microscope and used to obtain the best results. Each description is accompanied by photos of the various preparations observed with that kind of contrast, in some cases you can see the aesthetic differences taken from the same preparation, depending on the technique used.