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Microscopes > AXIOPHOT

Zeiss AXIOPHOT


SPECIFICATIONS
Oculars	PL 10x25mm
Objectives	ZEISS EC Plan-Neofluar 2,5x/0,075 oo/-0,17 ZEISS Ph1 Plan-NEOFLUAR 5x/0,15 oo/0,17 ZEISS Epiplan-NEOFLUAR 10x/0,30 HD DIC oo/0 ZEISS Ph2 Plan-NEOFLUAR 20x/0,50 oo/0,17 ZEISS Ph2 Plan-Neofluar 40x/0,75 oo/0,17 ZEISS Plan-NEOFLUAR 100x/1,30 oo/0,17
Nosepiece	5 Place DIC Nosepiece
Illumination	HBO 50W HBO 100W Halogen 100W
Optovar	Zoom 1-4x
Camera ports	35mm (2) 4x5 Video
Polarizer	Rotable 0-90°
Analyzer	Rotable
Filters	6 Position Fluorescence Block Filters


FILTERS SET BLOCK N.1
POSITION	BAND	Fluorochrome	EXCITATION	DICHROIC	EMISSION
1	Single	FITC, Fluorescein, Acridine Orange	485DF22	505DRLP	535DF35
2	Dual	Fluorescein, Acridine Orange Texas Red, Rhodamine B	490 577	490 575	528 633
3	Dual	CFP YFP	435/10 500/15	470/30 550/50	465/15 545/25
4	Dual	DAPI, HOECHST 33258, BFP Rhodamine B, TRITC, DsRed2	390 520	395 540	470 590
5	Single	DAPI, HOECHST 33258	330WB80	400DCLP	450DF65
6	Single	Spectrum Aqua	BP436/20	LP455	BP480/30


FILTERS SET BLOCK N.2
POSITION	BAND	Fluorochrome	EXCITATION	DICHROIC	EMISSION
1	Single	Spectrum Red	BP577/20	LP595	BP630/30
2	Single	Rhodamine B, TRITC, Texas Red	535DF35	570DRLP	590DF35
3	-	-	-	-	-

This is my current instrument, it is a ZEISS Axiophot 1, a direct descendant of the glorious ZEISS PHOTOMICROSCOPE III. This type of microscope, specifically designed for photomicrography, it is distinguished from other types because in them the Photomicrographic apparatus is constructed internally rather than being adapted as an accessory. This microscope, born in the late 80s, has the distinction of being modular, that can be assembled with various modules to have different configurations, among its main features, infinity objectives, can support different types of camera, Optovar by 1 to 4x zoom, remote control camera, fluorescence filter block with 7 positions.
The techniques are supported: Brightfield, Darkfield, Polarization, Phase Contrast, Episcopy, Epi-Fluorescence, DIC, COL, Rheinberg. It also allows the use of techniques such as composite: Epi-Fluorescence DIC, COL-Rheinberg, Epi-fluorescence-Phase Contrast, etc..
Video recording devices are: PAXCAM 2.0 Mp professional camera, compact camera SONY DSC-S85 4MP, compact camera Panasonic DMC-FZ8 12MP. Most of the photos of the site have been created through the use of this instrument with this type of devices.

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